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適用于體外泛素化
Quantity: 1 mg
MW: 8.8 kDa
Source: E. coli derived
Stock: Lyophilized from a solution in deionized water.
Solubility: Reconstitute at 10 mg/mL in an aqueous solution.
Purity: >95%, by SDSPAGE
Use: Recombinant Human Ubiquitin Mutant NoK can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin activating (E1) enzyme, a Ubiquitin conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Recombinant Human Ubiquitin Mutant NoK is unable to form chains, making it ideal for use as a negative control for chain formation or to confirm multimono ubiquitination of a substrate. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Mutant NoK concentration of 0.21mM.
Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
UbiquitinMutantNoK
UbiquitinMutantNoK
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